業績　Article

2017s：平成29年度

1．    Ueda K, Onishi A, Ito SI, Nakamura M, Takahashi M. Generation of three-dimensional retinal organoids expressing rhodopsin and S- and M-cone opsins from mouse stem cells. Biochem Biophys Res Commun. 2018 Jan 22;495(4):2595-2601.

2．  Kitahata S, Hirami Y, Takagi S, Kime C, Fujihara M, Kurimoto Y, Takahashi M.Efficacy of additional topical betamethasone in persistent cystoid macular oedema after carbonic anhydrase inhibitor treatments in retinitis pigmentosa. BMJ Open Ophthalmol. 2018 Jan 28;3(1):e000107.

3．  Takagi S, Hirami Y, Takahashi M, Yamamoto S, Goto S, Yamamoto M, Fujihara M, Tomita G, Kurimoto Y.Use of Wide-Field Fundus Camera, Fundus Autofluorescence, and OCT in Cases of Pigmented Paravenous Retinochoroidal Atrophy. Ophthalmol Retina. 2018 Jan;2(1):79-81.

4．  Kobayashi W, Onishi A, Tu HY, Takihara Y, Matsumura M, Tsujimoto K, Inatani M, Nakazawa T, Takahashi M.Culture Systems of Dissociated Mouse and Human Pluripotent Stem Cell-Derived Retinal Ganglion Cells Purified by Two-Step Immunopanning. Invest Ophthalmol Vis Sci. 2018 Feb 1;59(2):776-787.

5．  Iraha S, Tu HY, Yamasaki S, Kagawa T, Goto M, Takahashi R, Watanabe T, Sugita S, Yonemura S, Sunagawa GA, Matsuyama T, Fujii M, Kuwahara A, Kishino A, Koide N, Eiraku M, Tanihara H, Takahashi M, Mandai M.Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation. Stem Cell Reports. 2018 Mar 13;10(3):1059-1074.

2018s：平成30年度

1.       Sugita S, Makabe K, Iwasaki Y, Fujii S, Takahashi M.Natural Killer Cell Inhibition by HLA-E Molecules on Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells. Invest Ophthalmol Vis Sci. 2018 Apr 1;59(5):1719-1731.

2.     Goto S, Onishi A, Misaki K, Yonemura S, Sugita S, Ito H, Ohigashi Y, Ema M, Sakaguchi H, Nishida K, Takahashi M.Neural retina-specific Aldh1a1 controls dorsal choroidal vascular development via Sox9 expression in retinal pigment epithelial cells. Elife. 2018 Apr 3;7:e32358.

3.     Parmar T, Parmar VM, Perusek L, Georges A, Takahashi M, Crabb JW, Maeda A. Lipocalin 2 Plays an Important Role in Regulating Inflammation in Retinal Degeneration. J Immunol. 2018 May 1;200(9):3128-3141.

4.     Maeda A, Yoshida A, Kawai K, Arai Y, Akiba R, Inaba A, Takagi S, Fujiki R, Hirami Y, Kurimoto Y, Ohara O, Takahashi M.Development of a molecular diagnostic test for Retinitis Pigmentosa in the Japanese population. Jpn J Ophthalmol. 2018 Jul;62(4):451-457.

5.     Sugita S, Makabe K, Fujii S, Takahashi M.Detection of Complement Activators in Immune Attack Eyes After iPS-Derived Retinal Pigment Epithelial Cell Transplantation. Invest Ophthalmol Vis Sci. 2018 Aug 1;59(10):4198-4209.

6.     Takagi S, Hirami Y, Takahashi M, Fujihara M, Mandai M, Miyakoshi C, Tomita G, Kurimoto Y.Optical coherence tomography angiography in patients with retinitis pigmentosa who have normal visual acuity. Acta Ophthalmol. 2018 Aug;96(5):e636-e642.

7.     Takagi S, Mandai M, Hirami Y, Sugita S, Takahashi M, Kurimoto Y.Frequencies of human leukocyte antigen alleles and haplotypes among Japanese patients with age-related macular degeneration. Jpn J Ophthalmol. 2018 Sep;62(5):568-575.

8.     Fujii S, Yoshida S, Inagaki E, Hatou S, Tsubota K, Takahashi M, Shimmura S, Sugita S.Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells. Stem Cells Dev. 2019 Jan 1;28(1):28-43.

9.     Tu HY, Watanabe T, Shirai H, Yamasaki S, Kinoshita M, Matsushita K, Hashiguchi T, Onoe H, Matsuyama T, Kuwahara A, Kishino A, Kimura T, Eiraku M, Suzuma K, Kitaoka T, Takahashi M, Mandai M.Medium- to long-term survival and functional examination of human iPSC-derived retinas in rat and primate models of retinal degeneration. EBioMedicine. 2019 Jan;39:562-574. 

10.  Akiba R, Matsuyama T, Tu HY, Hashiguchi T, Sho J, Yamamoto S, Takahashi M, Mandai M.Quantitative and Qualitative Evaluation of Photoreceptor Synapses in Developing, Degenerating and Regenerating Retinas. Front Cell Neurosci. 2019 Feb 11;13:16.

11.    Kitahata S, Tanaka Y, Hori K, Kime C, Sugita S, Ueda H, Takahashi M.Critical Functionality Effects from Storage Temperature on Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cell Suspensions. Sci Rep. 2019 Feb 27;9(1):2891.

12.   Matsumoto E, Koide N, Hanzawa H, Kiyama M, Ohta M, Kuwabara J, Takeda S, Takahashi M.Fabricating retinal pigment epithelial cell sheets derived from human induced pluripotent stem cells in an automated closed culture system for regenerative medicine. PLoS One. 2019 Mar 13;14(3):e0212369.

13.   Jin ZB, Gao ML, Deng WL, Wu KC, Sugita S, Mandai M, Takahashi M.Stemming retinal regeneration with pluripotent stem cells. Prog Retin Eye Res. 2019 Mar;69:38-56.

14.  Hori K, Kuwabara J, Tanaka Y, Nishida M, Koide N, Takahashi M.A simple and static preservation system for shipping retinal pigment epithelium cell sheets. J Tissue Eng Regen Med. 2019 Mar;13(3):459-468.

2019s：令和元年度

1．    Makabe K, Sugita S, Hono A, Kamao H, Takahashi M.Mycoplasma Ocular Infection in Subretinal Graft Transplantation of iPS Cells-Derived Retinal Pigment Epithelial Cells. Invest Ophthalmol Vis Sci. 2019 Apr 1;60(5):1298-1308.

2．  Takagi S, Kudo S, Yokota H, Akiba M, Mandai M, Hirami Y, Takahashi M, Kurimoto Y, Ishida M.Assessment of the deformation of the outer nuclear layer in the Epiretinal membrane using spectral-domain optical coherence tomography. BMC Ophthalmol. 2019 May 17;19(1):113.

3．  An G, Akiba M, Yokota H, Motozawa N, Takagi S, Mandai M, Kitahata S, Hirami Y, Takahashi M, Kurimoto Y. Deep Learning Classification Models Built with Two-step Transfer Learning for Age Related Macular Degeneration Diagnosis. Annu Int Conf IEEE Eng Med Biol Soc. 2019 Jul;2019:2049-2052.

4．  Maeda A, Mandai M, Takahashi M.Gene and Induced Pluripotent Stem Cell Therapy for Retinal Diseases. Annu Rev Genomics Hum Genet. 2019 Aug 31;20:201-216.

5．  Kime C, Kiyonari H, Ohtsuka S, Kohbayashi E, Asahi M, Yamanaka S, Takahashi M, Tomoda K.Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells. Stem Cell Reports. 2019 Sep 10;13(3):485-498.

6．  Takagi S, Mandai M, Gocho K, Hirami Y, Yamamoto M, Fujihara M, Sugita S, Kurimoto Y, Takahashi M.Evaluation of Transplanted Autologous Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium in Exudative Age-Related Macular Degeneration. Ophthalmol Retina. 2019 Oct;3(10):850-859.

7．  Araki H, Miura F, Watanabe A, Morinaga C, Kitaoka F, Kitano Y, Sakai N, Shibata Y, Terada M, Goto S, Yamanaka S, Takahashi M, Ito T.Base-Resolution Methylome of Retinal Pigment Epithelial Cells Used in the First Trial of Human Induced Pluripotent Stem Cell-Based Autologous Transplantation. Stem Cell Reports. 2019 Oct 8;13(4):761-774.

8．  Kuwahara A, Yamasaki S, Mandai M, Watari K, Matsushita K, Fujiwara M, Hori Y, Hiramine Y, Nukaya D, Iwata M, Kishino A, Takahashi M, Sasai Y, Kimura T.Preconditioning the Initial State of Feeder-free Human Pluripotent Stem Cells Promotes Self-formation of Three-dimensional Retinal Tissue. Sci Rep. 2019 Dec 12;9(1):18936.

9．  Motozawa N, An G, Takagi S, Kitahata S, Mandai M, Hirami Y, Yokota H, Akiba M, Tsujikawa A, Takahashi M, Kurimoto Y.Optical Coherence Tomography-Based Deep-Learning Models for Classifying Normal and Age-Related Macular Degeneration and Exudative and Non-Exudative Age-Related Macular Degeneration Changes. Ophthalmol Ther. 2019 Dec;8(4):527-539.

10．          Ito SI, Takagi S, Takahashi M, Sugita S, Hirami Y, Fujihara M, Uzu T, Azumi A, Kurimoto Y.Bilateral retinitis after influenza virus infection in a case report. Am J Ophthalmol Case Rep. 2020 Jan 3;17:100584.

11． Kuwahara A, Yamasaki S, Mandai M, Watari K, Matsushita K, Fujiwara M, Hori Y, Hiramine Y, Nukaya D, Iwata M, Kishino A, Takahashi M, Sasai Y, Kimura T.Publisher Correction: Preconditioning the Initial State of Feeder-free Human Pluripotent Stem Cells Promotes Self-formation of Three-dimensional Retinal Tissue.Sci Rep. 2020 Feb 5;10(1):2237.

12．          Akiba R, Matsuyama T, Takahashi M, Mandai M. [Toward establishment of regenerative cell therapy for retinitis pigmentosa using iPS cell derived retinal sheet]. Nihon Yakurigaku Zasshi. 2020 Mar 1;155(2):93-98.

13．          Yang L, Fujinami K, Ueno S, Kuniyoshi K, Hayashi T, Kondo M, Mizota A, Naoi N, Shinoda K, Kameya S, Fujinami-Yokokawa Y, Liu X, Arno G, Pontikos N, Kominami T, Terasaki H, Sakuramoto H, Katagiri S, Mizobuchi K, Nakamura N, Mawatari G, Kurihara T, Tsubota K, Miyake Y, Yoshitake K, Iwata T, Tsunoda K; JEGC study group.Genetic Spectrum of EYS-associated Retinal Disease in a Large Japanese Cohort: Identification of Disease-associated Variants with Relatively High Allele Frequency. Sci Rep. 2020 Mar 26;10(1):5497.

14．          Singh MS, Park SS, Albini TA, Canto-Soler MV, Klassen H, MacLaren RE, Takahashi M, Nagiel A, Schwartz SD, Bharti K.Retinal stem cell transplantation: Balancing safety and potential. Prog Retin Eye Res. 2020 Mar;75:100779.

2020s：令和2年度

1．    Fujii S, Sugita S, Futatsugi Y, Ishida M, Edo A, Makabe K, Kamao H, Iwasaki Y, Sakaguchi H, Hirami Y, Kurimoto Y, Takahashi M.A Strategy for Personalized Treatment of iPS-Retinal Immune Rejections Assessed in Cynomolgus Monkey Models. Int J Mol Sci. 2020 Apr 27;21(9):3077.

2．  Matsuzaki M, Mandai M, Yamanari M, Totani K, Nishida M, Sugita S, Maeda T, Koide N, Takagi S, Hirami Y, Miyamoto N, Sugiyama S, Takahashi M, Kurimoto Y.Polarization-sensitive optical coherence tomography for estimating relative melanin content of autologous induced stem-cell derived retinal pigment epithelium. Sci Rep. 2020 May 6;10(1):7656.

3．  Fujinami-Yokokawa Y, Fujinami K, Kuniyoshi K, Hayashi T, Ueno S, Mizota A, Shinoda K, Arno G, Pontikos N, Yang L, Liu X, Sakuramoto H, Katagiri S, Mizobuchi K, Kominami T, Terasaki H, Nakamura N, Kameya S, Yoshitake K, Miyake Y, Kurihara T, Tsubota K, Miyata H, Iwata T, Tsunoda K; Japan Eye Genetics Consortium.Clinical and Genetic Characteristics of 18 Patients from 13 Japanese Families with CRX-associated retinal disorder: Identification of Genotype-phenotype Association. Sci Rep. 2020 Jun 12;10(1):9531.

4．  Sugita S, Mandai M, Hirami Y, Takagi S, Maeda T, Fujihara M, Matsuzaki M, Yamamoto M, Iseki K, Hayashi N, Hono A, Fujino S, Koide N, Sakai N, Shibata Y, Terada M, Nishida M, Dohi H, Nomura M, Amano N, Sakaguchi H, Hara C, Maruyama K, Daimon T, Igeta M, Oda T, Shirono U, Tozaki M, Totani K, Sugiyama S, Nishida K, Kurimoto Y, Takahashi M.HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration. J Clin Med. 2020 Jul 13;9(7):2217.

5．  Ye K, Takemoto Y, Ito A, Onda M, Morimoto N, Mandai M, Takahashi M, Kato R, Osakada F. Reproducible production and image-based quality evaluation of retinal pigment epithelium sheets from human induced pluripotent stem cells. Sci Rep. 2020 Sep 1;10(1):14387.

6．  Sugita S, Futatsugi Y, Ishida M, Edo A, Takahashi M.Retinal Pigment Epithelial Cells Derived from Induced Pluripotent Stem (iPS) Cells Suppress or Activate T Cells via Costimulatory Signals. Int J Mol Sci. 2020 Sep 5;21(18):6507.

7．  Makabe K, Sugita S, Mandai M, Futatsugi Y, Takahashi M.Microglia dynamics in retinitis pigmentosa model: formation of fundus whitening and autofluorescence as an indicator of activity of retinal degeneration. Sci Rep. 2020 Sep 7;10(1):14700.

8．  Inaba A, Maeda A, Yoshida A, Kawai K, Hirami Y, Kurimoto Y, Kosugi S, Takahashi M.Truncating Variants Contribute to Hearing Loss and Severe Retinopathy in USH2A-Associated Retinitis Pigmentosa in Japanese Patients. Int J Mol Sci. 2020 Oct 22;21(21):7817.

9．  Edo A, Sugita S, Futatsugi Y, Sho J, Onishi A, Kiuchi Y, Takahashi M.Capacity of Retinal Ganglion Cells Derived from Human Induced Pluripotent Stem Cells to Suppress T-Cells. Int J Mol Sci. 2020 Oct 22;21(21):7831.

10．          Yamanari M, Mase M, Obata R, Matsuzaki M, Minami T, Takagi S, Yamamoto M, Miyamoto N, Ueda K, Koide N, Maeda T, Totani K, Aoki N, Hirami Y, Sugiyama S, Mandai M, Aihara M, Takahashi M, Kato S, Kurimoto Y. Melanin concentration and depolarization metrics measurement by polarization-sensitive optical coherence tomography. Sci Rep. 2020 Nov 11;10(1):19513.

11． Yoshida A, Nakada H, Inaba A, Takahashi M.Ethical and professional challenges encountered by Japanese healthcare professionals who provide genetic counseling services. J Genet Couns. 2020 Dec;29(6):1004-1014.

12．          Uyama H, Mandai M, Takahashi M.Stem-cell-based therapies for retinal degenerative diseases: Current challenges in the establishment of new treatment strategies. Dev Growth Differ. 2021 Jan;63(1):59-71.

13．          Ishida M, Sugita S, Makabe K, Fujii S, Futatsugi Y, Kamao H, Yamasaki S, Sakai N, Maeda A, Mandai M, Takahashi M.A ROCK Inhibitor Promotes Graft Survival during Transplantation of iPS-Cell-Derived Retinal Cells. Int J Mol Sci. 2021 Mar 22;22(6):3237.

14．          Makabe K, Sugita S, Futatsugi Y, Takahashi M.Dynamics of Cyclooxygenase-1 Positive Microglia/Macrophage in the Retina of Pathological Model Mice as a Biomarker of the Retinal Inflammatory Diseases. Int J Mol Sci. 2021 Mar 25;22(7):3396.

2021s：令和3年度

1．    Ochiai K, Motozawa N, Terada M, Horinouchi T, Masuda T, Kudo T, Kamei M, Tsujikawa A, Matsukuma K, Natsume T, Kanda GN, Takahashi M, Takahashi K.A Variable Scheduling Maintenance Culture Platform for Mammalian Cells. SLAS Technol. 2021 Apr;26(2):209-217.

2．  Yamasaki S, Sugita S, Horiuchi M, Masuda T, Fujii S, Makabe K, Kawasaki A, Hayashi T, Kuwahara A, Kishino A, Kimura T, Takahashi M, Mandai M.Low Immunogenicity and Immunosuppressive Properties of Human ESC- and iPSC-Derived Retinas. Stem Cell Reports. 2021 Apr 13;16(4):851-867.

3．  Maeda T, Sugita S, Kurimoto Y, Takahashi M.Trends of Stem Cell Therapies in Age-Related Macular Degeneration. J Clin Med. 2021 Apr 20;10(8):1785.

4．  Matsuyama T, Tu HY, Sun J, Hashiguchi T, Akiba R, Sho J, Fujii M, Onishi A, Takahashi M, Mandai M.Genetically engineered stem cell-derived retinal grafts for improved retinal reconstruction after transplantation. iScience. 2021 Jul 16;24(8):102866.

5．  Sugita S, Mandai M, Kamao H, Takahashi M.Immunological aspects of RPE cell transplantation. Prog Retin Eye Res. 2021 Sep;84:100950.

6．  Fujinami-Yokokawa Y, Ninomiya H, Liu X, Yang L, Pontikos N, Yoshitake K, Iwata T, Sato Y, Hashimoto T, Tsunoda K, Miyata H, Fujinami K; Japan Eye Genetics Study (JEGC) Group.Prediction of causative genes in inherited retinal disorder from fundus photography and autofluorescence imaging using deep learning techniques. Br J Ophthalmol. 2021 Sep;105(9):1272-1279.

7．  Wu YR, Hashiguchi T, Sho J, Chiou SH, Takahashi M, Mandai M.Transplanted Mouse Embryonic Stem Cell-Derived Retinal Ganglion Cells Integrate and Form Synapses in a Retinal Ganglion Cell-Depleted Mouse Model. Invest Ophthalmol Vis Sci. 2021 Oct 4;62(13):26.

8．  Nishida M, Tanaka Y, Tanaka Y, Amaya S, Tanaka N, Uyama H, Masuda T, Onishi A, Sho J, Yokota S, Takahashi M, Mandai M.Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion. Sci Rep. 2021 Nov 2;11(1):21421.

9．  Sugita S, Hono A, Fujino S, Futatsugi Y, Yunomae Y, Shimizu N, Takahashi M.Detection of Mycoplasma Contamination in Transplanted Retinal Cells by Rapid and Sensitive Polymerase Chain Reaction Test. Int J Mol Sci. 2021 Nov 21;22(22):12555.

10．          Yamasaki S, Tu HY, Matsuyama T, Horiuchi M, Hashiguchi T, Sho J, Kuwahara A, Kishino A, Kimura T, Takahashi M, Mandai M.A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation. iScience. 2021 Dec 20;25(1):103657.

11． Yamasaki S, Kuwahara A, Kishino A, Kimura T, Takahashi M, Mandai M.Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture. Regen Ther. 2022 Jan 1;19:24-34.

12．          Motozawa N, Miura T, Ochiai K, Yamamoto M, Horinouchi T, Tsuzuki T, Kanda GN, Ozawa Y, Tsujikawa A, Takahashi K, Takahashi M, Kurimoto Y, Maeda T, Mandai M.Automated evaluation of retinal pigment epithelium disease area in eyes with age-related macular degeneration. Sci Rep. 2022 Jan 18;12(1):892.

13．          Woogeng IN, Kaczkowski B, Abugessaisa I, Hu H, Tachibana A, Sahara Y, Hon CC, Hasegawa A, Sakai N, Nishida M, Sanyal H, Sho J, Kajita K, Kasukawa T, Takasato M, Carninci P, Maeda A, Mandai M, Arner E, Takahashi M, Kime C. Inducing human retinal pigment epithelium-like cells from somatic tissue. Stem Cell Reports. 2022 Feb 8;17(2):289-306.

2022s：令和4年度

1．    Sakai D, Takagi S, Totani K, Yamamoto M, Matsuzaki M, Yamanari M, Sugiyama S, Yokota S, Maeda A, Hirami Y, Mandai M, Takahashi M, Nakamura M, Kurimoto Y.Retinal pigment epithelium melanin imaging using polarization-sensitive optical coherence tomography for patients with retinitis pigmentosa. Sci Rep. 2022 May 3;12(1):7115.

2．  Yamamoto T, Sato Y, Yasuda S, Shikamura M, Tamura T, Takenaka C, Takasu N, Nomura M, Dohi H, Takahashi M, Mandai M, Kanemura Y, Nakamura M, Okano H, Kawamata S.Correlation Between Genetic Abnormalities in Induced Pluripotent Stem Cell-Derivatives and Abnormal Tissue Formation in Tumorigenicity Tests. Stem Cells Transl Med. 2022 May 27;11(5):527-538.

3．  Maeda T, Mandai M, Sugita S, Kime C, Takahashi M.Strategies of pluripotent stem cell-based therapy for retinal degeneration: update and challenges. Trends Mol Med. 2022 May;28(5):388-404.

4．  Sakamoto T, Terasaki H, Yamashita T, Shiihara H, Funatsu R, Uemura A; Japanese Retina and Vitreous Society. Increased incidence of endophthalmitis after vitrectomy relative to face mask wearing during COVID-19 pandemic. Br J Ophthalmol. 2022 Jun 21:bjophthalmol-2022-321357.

5．  Kanda GN, Tsuzuki T, Terada M, Sakai N, Motozawa N, Masuda T, Nishida M, Watanabe CT, Higashi T, Horiguchi SA, Kudo T, Kamei M, Sunagawa GA, Matsukuma K, Sakurada T, Ozawa Y, Takahashi M, Takahashi K, Natsume T.Robotic search for optimal cell culture in regenerative medicine. Elife. 2022 Jun 28;11:e77007.

6．  Inaba A, Yoshida A, Maeda A, Kawai K, Kosugi S, Takahashi M.Perception of genetic testing among patients with inherited retinal disease: Benefits and challenges in a Japanese population. J Genet Couns. 2022 Aug;31(4):860-867.

7．  Uyama H, Tu HY, Sugita S, Yamasaki S, Kurimoto Y, Matsuyama T, Shiina T, Watanabe T, Takahashi M, Mandai M. Competency of iPSC-derived retinas in MHC-mismatched transplantation in non-human primates. Stem Cell Reports. 2022 Nov 8;17(11):2392-2408.